The purpose of this experiment was to take spinach leaves and extract the chlorophyll and carotenoid pigments by using acetone as the solvent. The chlorophyll and carotenoid pigments were extracted by using column chromography and alumina was used as the solvent. Solvents of different polarities were used, starting with the least polar, to extract the certain components from the leaves. They were then analyzed by using thin- layer chromatography.
Its tender, crispy, dark-green leaves are one of the favorite ingredients of chefs all around the world. Botanically, it belongs to the Amaranthaceae family, and its scientific name: Spinacia plant grows to about 1 foot in height.
Although it can be grown year round, its fresh greens are best available soon after the winter season from March through May in the Northern Hemisphere, and from September until November in the South of the equator.
Spinach plant with broad green leaves.
At least, two varieties of spinach are cultivated for their edible leaves; Savoy type with dark-green crinkle wrinkled leaves and flat-leaf variety with smooth surfaced leaves. Health Benefits of Spinach Spinach is storehouse for many phytonutrients that have health promotional and disease prevention properties.
It is very low in calories and fats g of raw leaves provide just 23 calories. Also, its leaves hold a good amount of soluble dietary fiber; no wonder why this leafy greens often recommended by dieticians in the cholesterol controlling and weight reduction programs!
Iron is an essential trace element required by the human body for red blood cell production and as a co-factor for an oxidation-reduction enzyme, cytochrome oxidase during the cellular metabolism.
Together, these compounds help act as protective scavengers against oxygen-derived free radicals and reactive oxygen species ROS that play a healing role in aging and various disease processes. Zeaxanthin, an important dietary carotenoid, is selectively absorbed into the retinal macula lutea in the eyes where it thought to provide antioxidant and protective UV light-filtering functions.
It thus helps protect from "age-related macular related macular disease" ARMDespecially in the older adults. Further, vitamin-A is required for maintaining healthy mucosa and skin and is essential for night vision. Consumption of natural vegetables and fruits rich in vitamin-A and flavonoids are also known to help the body protect from lung and oral cavity cancers.
Spinach leaves are an excellent source of vitamin-K. Vitamin-K plays a vital role in strengthening the bone mass by promoting osteoblastic activity in the bones. This green leafy vegetable also contains good amounts of many B-complex vitamins such as vitamin-B6 pyridoxinethiamin vitamin B-1riboflavin, folates, and niacin.
Folates help prevent neural tube defects in the newborns. Vitamin-C is a powerful antioxidant, which helps the body develop resistance against infectious agents and scavenge harmful oxygen-free radicals.
Its leaves also contain a good amount of minerals like potassium, manganese, magnesium, copper and zinc. Potassium is an important component of cell and body fluids that helps controlling heart rate and blood pressure. The human body uses manganese and copper as a co-factor for the antioxidant enzyme, superoxide dismutase.
Copper is also required for the production of red blood cells. Zinc is a co-factor for many enzymes that regulate growth and development, digestion and nucleic acid synthesis. It is also a small source of omega-3 fatty acids.
Regular consumption of spinach in the diet helps prevent osteoporosis weakness of bonesiron-deficiency anemia.
Moreover, its soft leaves are believed to protect the human body from cardiovascular diseases and cancers of colon and prostate. See the table below for in depth analysis of nutrients: Spinach nutrition Spinacia oleracearaw, values per g.The rate of photosynthesis was measured by the spinach disk method in which we replaced the air from the disks with sodium bicarbonate using a vacuum.
Under photosynthesis, oxygen, a Lab 4: Photosynthesis and Chromatography of Spinach Leaves Essay Lab 4: Photosynthesis and. Watch video · Once they had transformed the spinach leaf into a sort of mini heart, the team sent fluids and microbeads through its veins to show that blood cells can flow through this system.
Spinach leaf segments were infiltrated with % NaHCO3 and made to sink in solutions of % NaHCO3 so that rate of oxygen, production could be estimated . Flat- or smooth-leaf spinach has broad, smooth leaves that are easier to clean than Savoy.
This type is often grown for canned and frozen spinach, as well as soups, baby foods, and processed foods. Giant Noble is an example variety. Semi-savoy is a hybrid variety with slightly crinkled leaves.
It has the same texture as Savoy, but it is not as Family: Amaranthaceae.
Purpose: The purpose of this experiment was to take spinach leaves and extract the chlorophyll and carotenoid pigments by using acetone as the solvent. During the light reactions, pigments within the thylakoid membranes absorb solar energy, water is split, and oxygen is released.
(Mader, ) In this study, we are interested in examining the effects of photosynthesis in spinach leaves in the presence of different light conditions.